L-Selectin Defines a Bone Marrow Analogue to the Thymic Early T-Lineage Progenitor
نویسندگان
چکیده
The recent description of an early T-lineage progenitor (ETP) population in adult mouse thymus implies the presence of a bone marrow predecessor that has not yet been identified. Here we describe a Lin Neg Sca-1 Pos c-kit Hi Thy-1.1 Neg L-selectin Pos adult mouse bone marrow population that resembles the thymic ETP in both antigen expression phenotype and post-transplant lineage potential. These cells produce wave-like kinetics of thymic seeding, and reconstitute the irradiated thymus with kinetics comparable to a thymocyte graft after IV transplant. Transient B lineage reconstitution is also observed, but little myeloid potential can be detected in transplant experiments. A second subset of progenitors is L-selectin Neg and is highly enriched for rapid and persistent T and B lineage potential, as well as some myeloid potential. L-selectin (CD62L) is therefore an effective marker for separating lymphoid progenitors from myeloid progenitors and HSC in mouse bone marrow.
منابع مشابه
L-selectin defines a bone marrow analog to the thymic early T-lineage progenitor.
The recent description of an early T-lineage progenitor (ETP) population in adult mouse thymus implies the presence of a bone marrow predecessor that has not yet been identified. Here we describe a Lin(Neg) Sca-1(Pos) c-kit(Hi) Thy-1.1(Neg) L-selectin(Pos) adult mouse bone marrow population that resembles the thymic ETP in both antigen expression phenotype and posttransplantation lineage potent...
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